High‐Intensity Focused Ultrasound Ablation of Myocardium In Vivo and Instantaneous Biological Response

• DOI: 10.1111/echo.12526
• Publication Date: 2014-02-10T10:06:37Z
• Authors: Minjuan Zheng; Weihui Shentu; Dingzhang Chen; David J. Sahn; Xiaodong Zhou
• Keywords: High-intensity focused ultrasound; Eosin; Intensity

Objective This study aimed to evaluate the instantaneous biological response of canine myocardium in vivo high‐intensity focused ultrasound ( HIFU ) ablation, and thereby determine feasibility this method. Methods Left ventricle ablation was performed on six dogs at four levels energy (acoustic intensity 3000 W/cm 2 ; durations were 1.2, 2.4, 3.6, 4.8 sec, respectively). Gross lesion volumes confirmed assessed by tetrazolium chloride TTC staining, hematoxylin–eosin HE electron microscopy. Global cardiac function focal wall motion evaluated echocardiography. Blood enzymes troponin T CT nT) tested after ablation. repeated another set fresh hearts vitro same levels. Focal maximum temperatures detected both vitro. Results Different sizes via can be created beating using controlled emission. varied from 62 ± 4.8°C 81 12.9°C. The significantly smaller than vitro, as verified staining. immediately decreased (P < 0.05), although ejection fraction EF E/A ratio unchanged > 0.05). Enzymes nT increased. Conclusion used for controllable myocardial tissue, with instantly increased serum markers, regional motion, unaffected left ventricular global function.