Effects of florfenicol on the midgut physiological function of Bombyx mori, based on the diversity of intestinal microbiota

• DOI: 10.1111/eea.13486
• Publication Date: 2024-06-12T09:19:59Z
• Authors: Chao Tian; Hongbin Zou; Xiqian Guo; Qilong Shu; Xiaoxia Zhang; Jialu Cheng; Zhiya Gu; Fanchi Li; Bing Li
• Keywords: Bombycidae; Sericulture; Digestion

Abstract Florfenicol (FF) is a new antibiotic commonly used in sericulture. This study aimed to examine the effects of low (LC: 0.06 g/L), medium (MC: 0.12 and high (HC: 1.2 g/L) concentrations FF on midgut physiological functions silkworm, Bombyx mori (L.) (Lepidoptera: Bombycidae). The results showed that body weight whole cocoon silkworms decreased development duration fifth instar was prolonged HC group. structure intestinal microbiota silkworm larvae changed by exposure. Specifically, abundance conditional pathogens ( Curtobacterium , Sanguibater etc.) significantly increased, whereas Pseudomonas Pedobacter significantly. Additionally, reactive oxygen species level at 72 h elevated, muscle layer had become loose, together with appearance gut goblet cell atrophy. We observed increase Lactobacillus led reduction fluid pH, resulting α‐amylase protease activities, lipase activity exhibited an elevation. demonstrated accumulation peroxides induced exposure g/L caused damage, decrease pH from alterations microbial composition affected digestive enzyme activity, collectively leading impairment midgut. These findings provide valuable reference for safe use