Initial characterization of an unidentified Armillaria isolate from Serbia using LSU‐IGS1 and TEF‐1‐α genes

• DOI: 10.1111/efp.12135
• Publication Date: 2014-07-22T06:14:31Z
• Authors: N. Keča; N. B. Klopfenstein; M.‐S. Kim; H. Solheim; S. Woodward
• Keywords: Polyphyly; Intergenic region

Summary Armillaria species have a global distribution and play variable ecological roles, including causing root disease of diverse forest, ornamental horticultural trees. Accurate identification A rmillaria is critical to understand their roles. This work focused on characterizing an unidentified isolate from S erbian forest using pairing, sequencing the partial large subunit intergenic spacer‐1 regions r DNA ( LSU ‐ IGS 1) translation elongation factor‐1 alpha gene tef‐1α ) genes, phylogenetic analyses. Despite previously obtained 1 RFLP patterns that matched newly described N orth merican altimontana , pairing tests analyses sequences clearly demonstrate not . Based LSU‐IGS1, gallica isolates were polyphyletic, Serbian clustered with subset European within well‐supported clade (99%). appeared as separate, (97%) was basal other poorly resolved, polyphyletic clades containing E uropean isolates. It speculated f rom Serbia could represent evolutionary ancestral state because its position compared comprising Alternatively, this unusual hybrid high‐level sequence heterogeneity, represented by multiple two‐nucleotide codes, Further characterization needed confirm taxonomic status ecological/evolutionary significance unique, unknown erbia.