Comparative study of the expression of cholinergic system components in the CNS of experimental autoimmune encephalomyelitis mice: Acute vs remitting phase

• DOI: 10.1111/ejn.14125
• Publication Date: 2018-08-25T09:21:21Z
• Authors: Giovanni Di Pinto; Maria Di Bari; Rocío Martin‐Alvarez; Samantha Sperduti; Silvia Serrano‐Acedo; Valentina Gatta; Ada M. Tata; Guadalupe Mengod
• Keywords: Butyrylcholinesterase; Encephalomyelitis

Abstract Acetylcholine ( AC h) is involved in the modulation of inflammatory response. h levels are regulated by its synthesizing enzyme, choline acetyltransferase (Ch AT ), and hydrolyzing enzymes, mainly acetylcholinesterase hE) butyrylcholinesterase (BuChE). A more comprehensive understanding cholinergic system experimental autoimmune encephalomyelitis EAE ) disease progression could pave path for development therapies to ameliorate multiple sclerosis MS ). In this work, we analyzed possible alterations CNS neuroinflammation process using a MOG ‐induced mice model. ‐ vehicle‐treated animals were studied at acute remitting phases. We examined neuropathology mRNA expression Ch , hE α7 subunit nicotinic acetylcholine receptor (α7n hR), as well BuChE enzyme activities, brain spinal cord sections during progression. The activities these markers up‐ or down‐regulated many areas other phases disease. was present higher proportion astroglia microglia/macrophage cells group. observed changes cellular localization suggests their potential involvement neuroinflammatory may lay ground consider components putative anti‐inflammatory therapeutic targets MS.