MicroRNA‐27a‐3p relieves inflammation and neurologic impairment after cerebral ischaemia reperfusion via inhibiting lipopolysaccharide induced TNF factor and the TLR4/NF‐κB pathway

• DOI: 10.1111/ejn.15720
• Publication Date: 2022-05-18T23:09:11Z
• Authors: Jing Luo; Junjie Li; Li Xiong; Linna Fan; Lijia Peng; Yuan Yang; Di Lu; Jianlin Shao
• Keywords: Inflammation; Lipopolysaccharides; 0301 basic medicine; Interleukin-6; NF-kappa B; Apoptosis; Cerebral Infarction; Phosphoproteins; Brain Ischemia; Rats; Toll-Like Receptor 4; MicroRNAs; 03 medical and health sciences; Reperfusion; Animals; Nervous System Diseases

Abstract Cerebral ischaemia reperfusion (CIR) affects microRNA (miR) expression and causes substantial inflammation. Here, we investigated the influence underlying mechanism of miR‐27a‐3p in rats with CIR. First, biliverdin treatment relieved cerebral infarction decreased levels serum interleukin (IL)‐1β, IL‐6, TNF‐α. Through our previous study, found key its targeted gene LITAF might involve molecular Then, regulation between was verified by temporal profiles luciferase assay. Moreover, intracerebroventricular injection mimic significantly LITAF, TLR4, NF‐κB, IL‐6 at 24 h post‐surgery, whereas inhibitor reversed these effects. Furthermore, could relieve infarct neurologic deficit after In addition, neuronal damage induced Taken together, results suggest that protects against CIR relieving inflammation, damage, via regulating TLR4/NF‐κB pathway.