Tricalcium silicate cements: osteogenic and angiogenic responses of human bone marrow stem cells

• DOI: 10.1111/eos.12613
• Publication Date: 2019-04-08T20:25:48Z
• Authors: Mohamed R. W. Ali; Manal Mustafa; Asgeir Bårdsen; Athanasia Bletsa
• Keywords: Viability assay

Tricalcium silicate cements ( TSCs ) are used in endodontic procedures to promote wound healing and hard tissue formation. The aim of this study was evaluate compare the effect commonly [mineral trioxide aggregate MTA) , Biodentine, TotalFill] on cellular metabolism osteogenic/angiogenic differentiation human bone marrow mesenchymal stem cells hBMSCs vitro. We tested null hypothesis no difference between MTA TotalFill cell responses. Cells were subjected eluates materials for up 14 d. Cell viability evaluated using 3‐(4,5‐dimethyl‐thiazoyl)‐2,5‐diphenyl‐tetrazolium bromide MTT assay. Real‐time PCR determine levels expression osteogenic factors alkaline phosphatase ALP ), osteoprotegerin OPG osteocalcin OC collagen 1A COL1A1 angiogenic vascular endothelial growth factor A VEGF fibroblast 1 FGF ). ELISA s measure culture supernatants. Mineralization vitro assessed Alizarin Red staining. tolerated exposure well, with Biodentine showing most favorable viability. Expression differentially affected by materials, inducing earlier changes at gene level. Increased mineralization observed time, after all tested, greatest effect. results revealed different responses