Different parvalbumin and GABA expression in human epileptogenic focal cortical dysplasia

• DOI: 10.1111/epi.13405
• Publication Date: 2016-05-13T06:46:57Z
• Authors: Valentina Medici; Laura Rossini; Francesco Deleo; Giovanni Tringali; Laura Tassi; Francesco Cardinale; Manuela Bramerio; Marco de Curtis; Rita Garbelli; Roberto Spreafico
• Keywords: Immunostaining

Summary Objective Several studies have reported that inhibitory networks are altered in dysplastic tissue obtained from epilepsy surgery specimens. A consistent decrease the number of interneuronal subpopulation expresses parvalbumin ( PV ) was postsurgical patients with focal cortical dysplasia FCD ). We tested if protein expression observed epileptic corresponds to a parallel impairment γ‐aminobutyric acid GABA )ergic compartment. Methods analyzed 30 surgically treated who underwent for intractable including 26 (types I, II , and III 4 without any microscopic visible lesion (cryptogenic) as controls. Serial sections were processed using situ hybridization GAD ‐65 ‐67 probes immunocytochemistry antibody against . The density ‐immunoreactive interneurons relation ergic cells estimated controls all different pathologic groups by two‐ three‐dimensional (2D 3D) cell‐counting technique. Field fraction line profile analyses added estimate immunostaining proportion distribution signal generated gray matter. Results reduction ‐positive ‐immunoreactivity exclusively type I/ specimens compared cryptogenic control poor outcome. In profound rearrangement immunoreactivity observed, quantitative neurons terminals. did not reveal significant variations subtype. Significance Our study suggests preservation tissue, demonstrated substantial normal count neurons. selective is I an abnormal, but reduced, terminals confirmed Possible functional consequences discussed.