Suppressing evolution in genetically engineered systems through repeated supplementation

• DOI: 10.1111/eva.13119
• Publication Date: 2020-09-05T15:55:14Z
• Authors: Nathan C. Layman; Beth M. Tuschhoff; Andrew J. Basinski; Christopher H. Re...; James J. Bull; Scott L. Nuismer
• Keywords: Genetic Fitness

Genetically engineered organisms are prone to evolve in response the engineering. This evolution is often undesirable and can negatively affect purpose of Methods that maintain stability genomes therefore critical successful design use genetically organisms. One potential method limit unwanted by taking advantage ability gene flow counter local adaption, a process supplementation. Here, we investigate feasibility supplementation as mechanism offset evolutionary degradation transgene three model systems: bioreactor, drive, transmissible vaccine. In each model, continual introduction from stock used balance mutation selection against transgene. Each system has its unique features. The bioreactor especially tractable simple answer: level required at frequency