Single and repeated intra‐articular injections in the tarsocrural joint with allogeneic and autologous equine bone marrow‐derived mesenchymal stem cells are safe, but did not reduce acute inflammation in an experimental interleukin‐1β model of synovitis

• DOI: 10.1111/evj.13222
• Publication Date: 2019-12-10T17:39:59Z
• Authors: Aimée C. Colbath; Steven W. Dow; Leone S. Hopkins; Jennifer N. Phillips; C. Wayne McIlwraith; Laurie R. Goodrich
• Keywords: Joint effusion

Abstract Background Allogeneic and autologous bone marrow‐derived mesenchymal stem cells (BMDMSCs) have been administered in equine joints for their anti‐inflammatory effects. However, allogeneic BMDMSC offer multiple clinical practical advantages. Therefore, it is important to determine the relative effectiveness of vs BMDMSCs. Objectives The objective study was compare inflamed joint response BMDMSCs injections, if either treatment generated an effect. Study design Randomised controlled study. Method Bone marrow harvested from eight horses. Autologous pooled were culture expanded, cryopreserved thawed immediately prior administration. Ten million with 75 ng rIL‐1β into one tarsocrural contralateral received plus rIL‐1β. Repeat injections performed same joint. Four additional horses alone a single Clinical parameters (lameness, circumference effusion) synovial fluid parameters, including nucleated cell count (NCC), differential count, total protein (TP), prostaglandin E 2 (PGE ) C‐reactive (CRP), measured at baseline, 6, 12, 24, 72, 168 336 hours post‐injection. Results No difference detected between groups respect subjective lameness, effusion, circumference, NCC, TP, CRP or PGE . Neither nor treatments resulted improvement cytological over that elicited by alone. Main limitations A dose evaluated severe synovitis which may too observe BMDMSC‐mediated Conclusions This revealed equivalent response. equally ineffective reducing inflammatory acute rIL‐1β‐induced inflammation