The regenerative potential of fibroblasts in a new diabetes‐induced delayed humanised wound healing model

• DOI: 10.1111/exd.12097
• Publication Date: 2013-01-22T11:06:41Z
• Authors: Lucía Martínez‐Sa...; Claudio J. Conti; Sara Llames; Eva García; Luisa Retamosa; Almudena Holguín; Nuria Illera; Blanca Duarte; Lino Camblor; José M. Llaneza; José L. Jorcano; Fernando Larcher; Álvaro Meana; María J. Escámez; Marcela Del Río
• Keywords: Granulation tissue; Wound care

Abstract Cutaneous diabetic wounds greatly affect the quality of life patients, causing a substantial economic impact on healthcare system. The limited clinical success conventional treatments is mainly attributed to lack knowledge pathogenic mechanisms related chronic ulceration. Therefore, management ulcers remains challenging issue. Within this context, reliable animal models that recapitulate situations impaired wound healing have become essential. In study, we established new in vivo humanised model delayed context reproduces main features human disease. Diabetes was induced by multiple low doses streptozotocin bioengineered human‐skin‐engrafted immunodeficient mice. significant delay closure exhibited alterations granulation tissue formation and resolution, involving defects bed maturation, vascularisation, inflammatory response collagen deposition. model, cell‐based therapy consisting application plasma‐derived fibrin dermal scaffolds containing fibroblasts consistently improved triggering maturation further providing suitable matrix for migrating keratinocytes during re‐epithelialisation. present preclinical able shed light biological processes responsible improvement achieved, these findings can be extended designing therapeutic approaches with relevance.