A three‐dimensional skin equivalent reflecting some aspects of in vivo aged skin

• DOI: 10.1111/exd.12866
• Publication Date: 2015-10-06T15:23:20Z
• Authors: Johanna Diekmann; Lirija Alili; Okka Scholz; Melanie Giesen; Olaf Holtkötter; Peter Brenneisen
• Keywords: Skin equivalent; Skin Aging; Human skin; Epidermis (zoology); Dermal fibroblast

Abstract Human skin undergoes morphological, biochemical and functional modifications during the ageing process. This study was designed to produce a 3‐dimensional (3D) equivalent in vitro reflecting some aspects of vivo aged skin. Reconstructed generated by co‐culturing fibroblasts keratinocytes on collagen–glycosaminoglycan–chitosan scaffold, induced exposition Mitomycin‐C ( MMC ). Recently published data showed that treatment resulted drug‐induced accelerated senescence DIAS ) human dermal fibroblast cultures. Next established markers, histological changes were analysed comparison with In epidermis, filaggrin expression is reduced . Furthermore, tissue, amount elastin collagen lowered as well after 3D equivalents Our results show signs , which mimics