Long non‐coding RNA TSIX is upregulated in scleroderma dermal fibroblasts and controls collagen mRNA stabilization

• DOI: 10.1111/exd.12900
• Publication Date: 2015-11-14T08:22:36Z
• Authors: Zhongzhi Wang; Masatoshi Jinnin; Kayo Nakamura; Miho Harada; Hideo Kudo; Wakana Nakayama; Kuniko Inoue; Taiji Nakashima; Noritoshi Honda; Satoshi Fukushima; Hironobu Ihn
• Keywords: RNA Silencing

Long non-coding RNAs (lncRNAs) are thought to have various functions other than RNA silencing. We tried evaluate the expression of lncRNAs in patients with systemic sclerosis (SSc) and determined whether controls collagen dermal fibroblasts. lncRNA was by real-time PCR situ hybridization. Protein mRNA levels were analysed using immunoblotting PCR. found TSIX, one lncRNAs, overexpressed SSc fibroblasts both vivo vitro, which inhibited transfection transforming growth factor (TGF)-β1 siRNA. TSIX siRNA reduced type I normal fibroblasts, but not major disease-related cytokines. In addition, significantly stability, protein half-lives. Furthermore, we first investigated serum SSc, increased patients. is a new regulator stabilizes mRNA. The upregulation seen may result from activated endogenous TGF-β signalling play role constitutive these cells. Further studies on regulatory mechanism tissue fibrosis skin lead better understanding pathogenesis, diagnostic methods their therapeutic approaches siRNAs.