Platelet‐rich plasma improves the therapeutic efficacy of mesenchymal stem cells by enhancing their secretion of angiogenic factors in a combined radiation and wound injury model

• DOI: 10.1111/exd.14042
• Publication Date: 2019-09-28T02:48:40Z
• Authors: Hyunwook Myung; Hyosun Jang; Jae Kyung Myung; Changsun Lee; Janet Lee; JiHoon Kang; Won‐Suk Jang; Sun‐Joo Lee; Hyewon Kim; Hwi‐Yool Kim; Sunhoo Park; Sehwan Shim
• Keywords: Male; Vascular Endothelial Growth Factor A; Wound Healing; 0303 health sciences; Endothelin-1; Platelet-Rich Plasma; Neovascularization, Physiologic; Mesenchymal Stem Cells; Fetal Blood; Mesenchymal Stem Cell Transplantation; Urokinase-Type Plasminogen Activator; 3. Good health; Disease Models, Animal; Mice; 03 medical and health sciences; Culture Media, Conditioned; Paracrine Communication; Human Umbilical Vein Endothelial Cells; Animals; Humans; Signal Transduction

Delayed wound healing after radiation exposure can cause serious cutaneous damage, and its treatment is a major clinical challenge. Although mesenchymal stem cells (MSCs) have emerged as promising therapeutic agent in regenerative medicine, they alone do not produce satisfactory effects combined injury (CRWI) model. Here, we investigated the effect of umbilical cord blood-derived (UCB)-MSCs platelet-rich plasma (PRP) on CRWI mouse First, assessed release cytokines from UCB-MSCs cultured with PRP observed changes expression angiogenic factors. The paracrine factors were human vein endothelial (HUVECs). To assess efficacy, topically implanted into CRWT Vascular growth factor (VEGF), pro-angiogenic factor, urokinase-type plasminogen activator contributor to VEGF-induced signalling more highly expressed conditioned media than that alone. Furthermore, increased formation tube-like structures HUVECs. Co-treatment model closure rate angiogenesis compared an untreated irradiated group. Moreover, VEGF CD31 tissue co-treated mice mice. stimulates UCB-MSCs, therapy improves regeneration efficacy by enhancing