Investigation of miR‐126‐3p loaded on adipose stem cell‐derived exosomes for wound healing of full‐thickness skin defects

• DOI: 10.1111/exd.14480
• Publication Date: 2021-10-25T15:58:10Z
• Authors: Jie Ma; Zhaofeng Zhang; Yinmin Wang; Hua Shen
• Keywords: 0301 basic medicine; MicroRNAs; Wound Healing; 0303 health sciences; 03 medical and health sciences; Stem Cells; Human Umbilical Vein Endothelial Cells; Animals; Humans; Collagen; Exosomes; Cell Proliferation; Rats

AbstractObjectiveTo investigate the function of miR‐126‐3p loaded on adipose stem cell (ADSC)‐derived exosomes (ADSC‐Exos) in wound healing of full‐thickness skin defects.MethodsADSCs transfected with miR‐126‐3p mimic, miR‐126‐3p inhibitor or pcDNA3.1‐PIK3R2, or PKH26‐marked ADSC‐Exos were cultured with fibroblasts or human umbilical vein endothelial cells (HUVECs). The proliferation and migration rates of fibroblasts and angiogenesis of HUVECs were measured. Rats with full‐thickness skin defects were injected with ADSC‐Exos or exosomes extracted from ADSCs transfected with miR‐126‐3p inhibitor and the wound healing rates were measured. The wound bed, collagen deposition and angiogenesis in injured rats were assessed.ResultsADSC‐Exos could be ingested by fibroblasts and HUVECs. Co‐incubation with ADSCs or ADSC‐Exos promoted the proliferation and migration of fibroblasts and angiogenesis of HUVECs, which was further enhanced by miR‐126‐3p overexpression. Inhibition of ADSC‐Exos or miR‐126‐3p or PIK3R2 overexpression suppressed the proliferation and migration of fibroblasts and angiogenesis of HUVECs. ADSC‐derived exosomal miR‐126‐3p increased wound healing rate, collagen deposition and newly formed vessels in the injured rats.ConclusionADSC‐derived exosomal miR‐126‐3p promotes wound healing of full‐thickness skin defects by targeting PIK3R2.