Closing the gap: yeast electron‐transferring flavoprotein links the oxidation of d‐lactate and d‐α‐hydroxyglutarate to energy production via the respiratory chain

• DOI: 10.1111/febs.14924
• Publication Date: 2019-05-13T08:37:30Z
• Authors: Marina Toplak; Julia Brunner; Chaitanya R. Tabib; Peter Macheroux
• Keywords: Flavoprotein; Electron acceptor; Mitochondrial respiratory chain

Electron-transferring flavoproteins (ETFs) have been found in all kingdoms of life, mostly assisting shuttling electrons to the respiratory chain for ATP production. While human (h) ETF has studied great detail, very little is known about biochemical properties homologous protein model organism Saccharomyces cerevisiae (yETF). In view absence client dehydrogenases, example, acyl-CoA dehydrogenases involved β-oxidation fatty acids, d-lactate dehydrogenase 2 (Dld2) appeared be only relevant enzyme that serviced by yETF electron transfer mitochondrial transport chain. However, this hypothesis was never tested experimentally. Here, we report and Dld2 as well reaction between two proteins. Our study revealed oxidizes d-α-hydroxyglutarate more efficiently than exhibiting k