Mechanism of transcription regulation by Acinetobacter baumannii HpaR in the catabolism of p‐hydroxyphenylacetate

• DOI: 10.1111/febs.16340
• Publication Date: 2021-12-30T12:05:52Z
• Authors: Permkun Permsiriv...; Anan Yuenyao; Nuttawan Pramanpol; Ratana Charoenwat...; Wipa Suginta; Pimchai Chaiyen; Danaya Pakotiprapha
• Keywords: Transcription; Acinetobacter baumannii

HpaR is a transcription regulator in the MarR family that controls expression of gene cluster responsible for conversion p ‐hydroxyphenylacetate to pyruvate and succinate cellular metabolism. Here, we report biochemical structural characterization Acinetobacter baumannii ( Ab HpaR) its complex with cognate DNA. Our study revealed binds upstream divergently transcribed hpaA meta ‐cleavage operon, as well hpaR gene, thereby repressing their by blocking access RNA polymerase. Structural analysis HpaR‐DNA DNA binding specificity can be achieved via combination both direct indirect sequence readouts. weakened 3,4‐dihydroxyphenylacetate (DHPA), which substrate meta‐ cleavage reactions; this likely leads target genes. Based on our findings, propose model how A. HPA‐metabolizing genes, highlights independence global catabolite repression could beneficial metabolic engineering toward bioremediation applications. Databases The data support these findings are openly available wwPDB at https://doi.org/10.2210/pdb7EL2/pdb https://doi.org/10.2210/pdb7EL3/pdb complex, respectively.