Fine‐tuned calcium homeostasis is crucial for murine erythropoiesis

• DOI: 10.1111/febs.17401
• Publication Date: 2025-01-22T05:36:41Z
• Authors: Shujing Zhang; Yuanzhen Zhang; Yanxia Li; Zhiyue Zhang; Hui Li; Miaomiao Xu; Zhiyuan Lu; Yuan Li; Baobing Zhao
• Keywords: Ionomycin; Calcium in biology; Calcium Signaling

Intracellular calcium (Ca 2+ ) is a crucial signaling molecule involved in multiple cellular processes. However, the functional role of Ca terminal erythropoiesis remains unclear. Here, we uncovered dynamics intracellular levels during mouse erythroid development. By using ionophore ionomycin, found that low are required for expansion progenitors, whereas higher led to differentiation and proliferation early‐stage erythroblasts. were then gradually reduced, which nuclear condensation polarisation at late stage differentiation. elevated late‐stage erythroblasts, achieved by promoted enucleation via calmodulin (CaM)/calcium/calmodulin‐dependent protein kinase 1 (CaMKK1)/AMPK signaling. These data suggest reduction plays double‐edged differentiation, beneficial but compromises enucleation. Our study highlighted importance fine‐tuned regulation erythropoiesis, providing cues efficient generation mature enucleated erythrocytes vitro .