Increased Outer Membrane Vesicle Formation in a Helicobacter pylori tolB Mutant

• DOI: 10.1111/hel.12196
• Publication Date: 2015-02-09T09:15:19Z
• Authors: Lorinda Turner; Judyta Praszkier; Melanie L. Hutton; David Steer; Georg Ramm; Maria Kaparakis‐L...; Richard L. Ferrero
• Keywords: Pathogenicity island; Cell envelope

Multiple studies have established the importance of tol-pal gene cluster in bacterial cell membrane integrity and outer vesicle (OMV) formation Escherichia coli. In contrast, functions Tol-Pal proteins pathogenic organisms, including those Epsilonproteobacteria, remain poorly if at all defined. The aim this study was to characterize roles two key components system, TolB Pal, OMV bacterium, Helicobacter pylori.H. pylori ΔtolB, Δpal ΔtolBpal mutants, as well complemented strains, were generated assessed for changes morphology production by scanning electron microscopy enzyme-linked immunoassay (ELISA), respectively. protein content pro-inflammatory properties OMVs determined mass spectroscopy interleukin-8 (IL-8) ELISA on culture supernatants from OMV-stimulated cells, respectively.H. ΔtolB bacteria exhibited aberrant and/or flagella biosynthesis. Importantly, disruption H. tolB but not pal resulted a significant increase production. harbored many major virulence observed wild-type (WT) OMVs. Interestingly, induced significantly higher levels IL-8 host compared with WT OMVs.This work demonstrates that Pal are important pylori. Moreover, it shows how tolB-pal genes may be manipulated develop "hypervesiculating" strains vaccine purposes.