Immunohistochemistry for trimethylated H3K27 in the diagnosis of malignant peripheral nerve sheath tumours

• DOI: 10.1111/his.13072
• Publication Date: 2016-08-31T18:40:06Z
• Authors: Naofumi Asano; Akihiko Yoshida; Hitoshi Ichikawa; Taisuke Mori; Masaya Nakamura; Akira Kawai; Nobuyoshi Hiraoka
• Keywords: Immunostaining; Polyclonal antibodies

Aims The diagnosis of a malignant peripheral nerve sheath tumour ( MPNST ) can be challenging, as the morphological criteria and existing immunohistochemical markers are not entirely specific. recent discovery frequent inactivation polycomb repressive complex 2 in s suggests that detection histone 3 trimethylated on lysine 27 (H3K27me3) could diagnostic help. This study aimed to clarify utility this marker. Methods results We performed immunostaining studies, with monoclonal (C36B11) polyclonal antibodies parallel. With antibody, 56% 54 conventional showed complete loss staining, whereas 17% mosaic 28% intact staining. Three novel geographical pattern loss. All three epithelioid retained Among 232 non‐ s, only two (0.9%) Mosaic was observed 38% remaining 61% For H3K27me3 significantly associated higher TNM stage P = 0.013), deeper location 0.004), presence heterologous differentiation 0.003). Polyclonal did recognize 34% cases use antibodies. Conclusions confirmed staining is moderately sensitive highly specific for s. In contrast prior we found non‐specific, caution such should considered diagnostic. recommend antibody obtain better performance.