Detection of the unknown components of the oral microflora of teeth with periapical radiolucencies in a Turkish population using next‐generation sequencing techniques

• DOI: 10.1111/iej.12956
• Publication Date: 2018-06-01T07:06:03Z
• Authors: E. İriboz; B. Arıcan Öztürk; M. Kolukırık; İ. Karacan; H. Sazak Öveçoğlu
• Keywords: Sodium hypochlorite

Abstract Aim To detect the unknown components of oral microbiome and effects root canal treatment in a Turkish population to evaluate changes microbial diversity canals before after treatment. Methodology Single‐rooted central lateral maxillary incisors with one were chosen from 20 patients. Baseline samples intact intracanal microbiota collected permanent teeth necrotic pulps using sterile paper points. After preparation, filled calcium hydroxide paste for 7 days. Calcium was removed 2.5% sodium hypochlorite 17% EDTA passive ultrasonic irrigation ( PUI ). A second bacteriologic taken points prior filling. The processes DN ase‐I next‐generation sequencing NGS Reduction bacterial numbers during evaluated real‐time quantitative PCR qPCR All statistical analyses conducted MINITAB 17 software (Minitab Ltd. Co., Coventry, UK). one‐sample t ‐test used analyse data. Statistical significance accepted at P < 0.05. Results Relative abundances Mycoplasma sp., Paludibacter Tannerella Prevotella spp. an uncultured species order Bacteroidales decreased preparation medication (98.7%, 99.8%, 98.8%, 97.7% 99.3%, respectively), whilst relative Methylobacterium Corynebacterium sp. Streptococcus infantis increased (93.1%, 94.8% 99.4%, respectively). Considerable Streptophyta detected ratio Agrobacterium treated community infected had negative correlations success elimination. Conclusions combination techniques resulted detection previously on their abundance population.