Histological evaluation of a novel phosphorylated pullulan‐based pulp capping material: An in vivo study on rat molars

• DOI: 10.1111/iej.13587
• Publication Date: 2021-06-07T09:18:12Z
• Authors: Rafiqul Islam; Yu Toida; Fei Chen; Toru Tanaka; Satoshi Inoue; Tetsuya Kitamura; Yasuhiro Yoshida; Abu Faem Mohammad...; Hany Mohamed Aly ...; Hidehiko Sano
• Keywords: Pullulan; Pulp capping

Abstract Aim To evaluate the dental pulp response to a novel mineral trioxide aggregate containing phosphorylated pullulan (MTAPPL) in rats after direct capping. Methods Ninety‐six cavities were prepared maxillary first molars of 56 male Wistar rats. The pulps intentionally exposed and randomly divided into four groups according application capping materials: MTAPPL; (PPL); conventional MTA (Nex‐Cem MTA, NCMTA; positive control); Super‐Bond (SB; negative control). All restored with SB observed for pulpal responses at 1‐, 3‐, 7‐ 28‐day intervals using histological scoring system. Statistical analysis was performed Kruskal–Wallis Mann–Whitney U ‐test Bonferroni's correction, level significance set 0.05. DMP1 CD34 antigen used odontoblast differentiation vascularization, respectively. Results On day 1, mild inflammatory cells present MTAPPL NCMTA groups; fewer PPL, whereas associated mild‐to‐moderate response. A significant difference between PPL ( p < .05). No mineralized tissue deposition observed. 3, moderate‐to‐severe SB, had Initial NCMTA, SB. 7, thin layer all tested no or 28, MTAPPL, responses. Complete barrier formation > exhibited incomplete barriers, significantly different from staining on observation days. Conclusion favourable induction indicate its potential as material.