Effects of physicochemical factors and in vitro gastrointestinal digestion on antioxidant activity of R‐phycoerythrin from red algae Bangia fusco‐purpurea

• DOI: 10.1111/ijfs.12775
• Publication Date: 2015-03-02T13:30:59Z
• Authors: Qiang Wu; Xiao‐Ping Fu; Le‐Chang Sun; Qian Zhang; Guang‐Ming Liu; Min‐Jie Cao; Qiu‐Feng Cai
• Keywords: ABTS; Digestion; Scavenging; Phycoerythrin; Hydroxyl radical

Summary R ‐phycoerythrin ( ‐ PE ) was purified from the red algae B angia fusco‐purpurea after 35–50% ammonium sulphate fractionation followed by ion‐exchange column chromatography on DEAE‐Sepharose, resulting in a purity (A 565 /A 280 ratio of 5.1. The circular dichroism spectroscopy results suggested that structure R‐PE is predominately helical. antioxidant activity studied and revealed changes conformation at different temperatures pH values. After vitro ‐simulated gastrointestinal (GI) digestion R‐PE, scavenging ABTS radical (EC 50 , 769.9 μg mL −1 ), DPPH 421.9 hydroxyl 32.4 reducing power 700 = 0.5, 625.8 were measured. Gel filtration analysis showed molecular weight distribution final GI digest still contained high <3 kD a. Our present indicate digestion‐resistant peptides may be obtained proteinases degradation.