Comparative metabolite profiling of raw milk from subclinical and clinical mastitis cows using 1H‐NMR combined with chemometric analysis

• DOI: 10.1111/ijfs.14665
• Publication Date: 2020-06-08T13:03:14Z
• Authors: Mayuree Luangwilai; Kiattisak Duangmal; Nawanon Chantapra...; Sarn Settachaimon...
• Keywords: Isoleucine

Summary The objectives of this study were to characterise and compare non‐volatile metabolite profiles raw milk from healthy, subclinical clinical mastitis cows using a non‐targeted proton nuclear magnetic resonance ( 1 H‐NMR) metabolomic approach. A total 46 metabolites identified. Multivariate analysis allowed discrimination healthy according their H‐NMR profiles. Significant increases in acetate, formate, lactate, benzoate, hippurate, β‐hydroxybutyrate, valerate, alanine, histidine, isoleucine, leucine, N ‐acetylamino acid, phenylalanine, threonine valine content associated with extremely high somatic cell count status the cows. Furthermore, significant rises ‐acetylglucosamine, isoleucine leucine could be used as potential biomarker for indicating infection udders. Beside existing biomarkers diagnosing literatures, we found additional candidate metabolites, such valerate correlation Thai crossbred Holstein dairy