The influence of agents differentiating HL‐60 cells toward granulocyte‐like cells on their ability to release neutrophil extracellular traps

• DOI: 10.1111/imcb.12015
• Publication Date: 2018-01-30T14:37:54Z
• Authors: Aneta Manda‐Handzlik; Weronika Bystrzycka; Małgorzata Wachowska; Sandra Sieczkowska; Anna Stelmaszczyk...; Urszula Demkow; Olga Ciepiela
• Keywords: Neutrophil Extracellular Traps

Abstract Studies on neutrophil extracellular traps ( NET s) are challenging as neutrophils live shortly and easily become activated. Thus, availability of a cell line model closely resembling the functions peripheral blood would be advantageous. Our purpose was to find compound that most effectively differentiates human promyelocytic leukemia (HL‐60) cells toward granulocyte‐like able release s. HL ‐60 were differentiated with all‐trans retinoic acid ATRA ), dimethyl sulfoxide DMSO ) or dimethylformamide DMF stimulated phorbol 12‐myristate 13‐acetate PMA calcium ionophore A23187 CI ). Cell differentiation, phagocytosis influx analyzed by flow cytometry. Reactive oxygen species production s measured fluorometrically microscopically. LC 3‐ II accumulation histone 3 citrullination western blot. cells. ‐ dHL released only upon stimulation, post while formed in response both stimuli. Oxidative burst induced ‐, stimulation stimulation. Increased observed but not The diminished Significant increase autophagosomes formation ‐stimulated Phagocytic index higher than control, We conclude , differentiate different mechanisms. is best stimulus for differentiation NETs studies.