Epstein–Barr virus (EBV) provides survival factors to EBV+ diffuse large B‐cell lymphoma (DLBCL) lines and modulates cytokine induced specific chemotaxis in EBV+ DLBCL

• DOI: 10.1111/imm.12792
• Publication Date: 2017-07-12T05:12:39Z
• Authors: Liang Wu; Barbro Ehlin‐Henr...; Xiaoying Zhou; Hong Zhu; Ingemar Ernberg; Lorand L. Kis; George Klein
• Keywords: 0301 basic medicine; Epstein-Barr Virus Infections; Herpesvirus 4, Human; Receptors, CCR7; Receptors, CXCR4; Cell Survival; Chemotaxis; Neoplasm Proteins; 3. Good health; 03 medical and health sciences; Cell Line, Tumor; Tumor Microenvironment; Cytokines; Humans; Lymphoma, Large B-Cell, Diffuse

Diffuse large B-cell lymphoma (DLBCL), the most common type of malignant lymphoma, accounts for 30% adult non-Hodgkin lymphomas. Epstein-Barr virus (EBV) -positive DLBCL elderly is a newly recognized subtype that 8-10% DLBCLs in Asian countries, but less Western populations. Five DLBCL-derived cell lines were employed to characterize patterns EBV latent gene expression, as well response cytokines and chemotaxis. Interleukin-4 interleukin-21 modified LMP1, EBNA1 EBNA2 expression depending on phenotype programme (type I, II or III). These also affected CXCR4- CCR7-mediated chemotaxis two lines, Farage III) Val II). Further, we investigated effect by using dominant-negative nuclear antigen 1(dnEBNA1) eliminate genomes. This resulted decreased By employing an alternative way genomes, Roscovitine, show increase apoptosis EBV-positive lines. results plays important role with regard survival chemotactic response. Our findings provide evidence impact microenvironment EBV-carrying cells might have therapeutic implications.