Regulatory T‐cells in acute dengue viral infection

• DOI: 10.1111/imm.12863
• Publication Date: 2017-11-15T14:32:40Z
• Authors: Hemadri Eresha Ja...; Dulharie Wijeratne; Samitha Fernando; Achala Kamaladasa; Laksiri Gomes; Ananda Wijewickrama; Graham Stuart Ogg; Gathsaurie Neelik...
• Keywords: ELISPOT; Regulatory T cell; Ex vivo

Summary Although regulatory T‐cells (T regs ) have been shown to be expanded in acute dengue, their role pathogenesis and relationship clinical disease severity extent of viraemia not fully evaluated. The frequency T was assessed 56 adult patients with dengue by determining the proportion forkhead box protein 3 (FoxP3) expressing CD4 + CD25 (FoxP3 cells). Dengue virus (DENV) viral loads were measured quantitative real‐time polymerase chain reaction (PCR) DENV‐specific T‐cell responses ex‐vivo interferon (IFN)‐ γ enzyme‐linked immunospot (ELISPOT) assays overlapping peptide pools DENV‐NS3, NS1 NS5. CD45RA CCR4 used phenotype different subsets suppressive potential expression cytotoxic lymphocyte‐antigen 4 (CTLA‐4) Fas. While FoxP3 cells significantly higher ( P < 0·0001) when compared healthy individuals, they did show any or degree viraemia. correlate either IFN‐ DENV‐NS3‐, NS5‐ NS1‐specific responses. predominantly low , followed CD45RA‐FoxP3 only a small being highly effector Treg subtype. Expression also majority T‐cells, expressed at high levels reg population. Therefore, although are consist naive poor capacity.