MicroRNA signature of central nervous system‐infiltrating dendritic cells in an animal model of multiple sclerosis

• DOI: 10.1111/imm.12934
• Publication Date: 2018-05-11T06:11:16Z
• Authors: Mariah L. Hoye; Angela S. Archamb...; Taylor M. Gordon; Landon K. Oetjen; Matthew D. Cain; Robyn S. Klein; Seth D. Crosby; Brian S. Kim; Timothy M. Miller; Gregory F. Wu
• Keywords: CD11c

Summary Innate immune cells are integral to the pathogenesis of several diseases central nervous system (CNS), including multiple sclerosis (MS). Dendritic (DCs) potent CD11c + antigen‐presenting that critical regulators adaptive responses, particularly in autoimmune such as MS. The regulation DC function both periphery and CNS compartment has not been fully elucidated. One limitation studying role DCs is microglia can upregulate during inflammation, making it challenging distinguish bone marrow‐derived (BMDCs) from microglia. Selective expression microRNAs (miRNAs) shown populations innate regulate their within neuro‐inflammation. Using experimental encephalomyelitis (EAE) murine model MS, we characterized miRNAs using a non‐biased array. Several miRNAs, miR‐31, were enriched EAE, but LysM Moreover, CD11c, generated marrow chimeras found miR‐31 was specific BMDCs. Interestingly, miR‐31‐binding sites mRNAs downregulated BMDCs migrated into CNS, subset confirmed be regulated by miR‐31. Finally, elevated migrating through an vitro blood–brain barrier. Our findings suggest may entry could potentially represent therapeutic targets for