Interleukin‐6 signalling mediates Galectin‐8 co‐stimulatory activity of antigen‐specific CD4 T‐cell response

• DOI: 10.1111/imm.12980
• Publication Date: 2018-07-04T17:53:22Z
• Authors: Julieta Carabelli; Cecilia A. Prato; Liliana M. Sanmarco; Maria P. Aoki; Oscar Campetella; María V. Tribulatti
• Keywords: Co-stimulation

Summary Galectin‐8 (Gal‐8) is a mammalian lectin endowed with the ability to co‐stimulate antigen‐specific immune responses. We have previously demonstrated that bone‐marrow‐derived dendritic cells produce high levels of interleukin‐6 ( IL ‐6) in response Gal‐8 stimulation. As ‐6 pleiotropic cytokine has broad effect on system, we aimed elucidate whether was involved Gal‐8‐dependent co‐stimulatory signals during antigen recognition by specific CD 4 T cells. With this aim, splenocytes from DO 11.10 mice were incubated low dose cognate ovalbumin peptide combination Gal‐8. Interleukin‐6 found significantly increased cultures stimulated alone or plus peptide. Moreover, signalling triggered Gal‐8‐induced co‐stimulation, as determined phosphorylation signal transducer and activator transcription 3. blockade neutralizing monoclonal antibody precluded activity but did not affect ‐cell receptor activation. Different subsets cells, well macrophages B identified cellular source co‐stimulation. To confirm mediated effect, antigen‐presenting ‐6‐deficient wild‐type co‐cultured purified OTII presence Notably, response, impaired In addition, exogenous fully restored Taken together, our results demonstrate mediates immune‐stimulatory effect.