Transcriptomic profiling of cardiac tissues from SARS‐CoV‐2 patients identifies DNA damage

• DOI: 10.1111/imm.13577
• Publication Date: 2022-09-15T15:59:02Z
• Authors: Arutha Kulasinghe; Ning Liu; Chin Wee Tan; James Monkman; Jane E. Sinclair; Dharmesh D. Bhuva; David Godbolt; Liuliu Pan; Andy Nam; Habib Sadeghirad; Kei Sato; Gianluigi Li Bassi; Ken O'Byrne; Camila Hartmann; Anna Flavia Ribei...; Gustavo Lenci Mar...; Lidia Zytynski Moura; Derek Richard; Mark Adams; Lucia de Noronha; Cristina Pellegri...; Jacky Y. Suen; Rakesh Arora; Gabrielle T. Belz; Kirsty R. Short; Melissa J. Davis; Fernando Souza‐Fo...; John F. Fraser
• Keywords: Profiling (computer programming)

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is known to present with pulmonary and extra-pulmonary organ complications. In comparison the 2009 pandemic (pH1N1), SARS-CoV-2 infection likely lead more disease, multi-organ effects, including cardiovascular disease. has been associated long-term but molecular changes that govern this remain unknown. study, we investigated host transcriptome landscape of cardiac tissues collected at rapid autopsy from seven SARS-CoV-2, two pH1N1, six control patients using targeted spatial transcriptomics approaches. Although was not detected in tissue, showed upregulation genes DNA damage repair, heat shock, M1-like macrophage infiltration COVID-19 patients. patient samples, were further confirmed by γ-H2Ax immunohistochemistry. comparison, pH1N1 interferon-stimulated genes, particular interferon complement pathways, when compared These data demonstrate emergence distinct transcriptomic profiles influenza supporting need for a greater understanding effects on organs, system patients, delineate immunopathobiology infection, long term impact health.