CXCR3+ effector regulatory T cells associate with disease tolerance during lower respiratory pneumovirus infection

• DOI: 10.1111/imm.13790
• Publication Date: 2024-04-08T01:39:30Z
• Authors: Ismail Sebina; Sylvia Ngo; Ridwan B. Rashid; Mariah Alorro; Patricia Namubiru; Daniel Howard; Tufael Ahmed; Simon Phipps
• Keywords: CXCR3; CXCL9

Lifestyle factors like poor maternal diet or antibiotic exposure disrupt early life microbiome assembly in infants, increasing the risk of severe lower respiratory infections (sLRI). Our prior studies mice indicated that a low-fibre (LFD) exacerbates LRI severity infants by impairing recruitment plasmacytoid dendritic cells (pDC) and consequently attenuating expansion lung regulatory T (Treg) during pneumonia virus (PVM) infection. Here, we investigated whether dietary fibre intake influences Treg cell phenotypes mediastinal lymph nodes (mLN) lungs PVM-infected neonatal mice. Using high dimensional flow cytometry, identified distinct clusters (Treg cells), which differed between mLN infection, with notably greater effector accumulation lungs. Compared to high-fibre (HFD)-reared pups, frequencies various subsets were decreased LFD-reared pups. Particularly, chemokine receptor 3 (CXCR3