Acute and chronic wound fluids influence keratinocyte function differently

Adult Aged, 80 and over Keratinocytes Male Pressure Ulcer 0301 basic medicine Abdominoplasty Cell Culture Techniques Exudates and Transudates Middle Aged Fibroblast Growth Factors 03 medical and health sciences 0302 clinical medicine Matrix Metalloproteinase 9 Cell Movement Acute Disease Chronic Disease Humans Female Matrix Metalloproteinase 3 Cells, Cultured Aged Cell Proliferation
DOI: 10.1111/iwj.12069 Publication Date: 2013-03-21T08:35:21Z
ABSTRACT
AbstractWound healing requires a proper functioning of keratinocytes that migrate, proliferate and lead to a competent wound closure. Impaired wound healing might be due to a disturbed keratinocyte function caused by the wound environment. Basically, chronic wound fluid (CWF) differs from acute wound fluid (AWF). The aim of this study was to analyse the effects of AWF and CWF on keratinocyte function. We therefore investigated keratinocyte migration and proliferation under the influence of AWF and CWF using MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] test and scratch assay. We further measured the gene expression by qRT‐PCR regarding growth factors and matrixmetalloproteinases (MMPs) involved in regeneration processes. AWF had a positive impact on keratinocyte proliferation over time, whereas CWF had an anti‐proliferative effect. Keratinocyte migration was significantly impaired by CWF in contrast to an undisturbed wound closure under the influence of AWF. MMP‐9 expression was strongly upregulated by CWF compared with AWF. Keratinocyte function was significantly impaired by CWF. An excessive induction of MMP‐9 by CWF might lead to a permanent degradation of extracellular matrix and thereby prevent wounds from healing.
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