Growth factor‐independent 1B gene (GFI1B) is overexpressed in erythropoietic and megakaryocytic malignancies and increases their proliferation rate

• DOI: 10.1111/j.1365-2141.2006.06407.x
• Publication Date: 2006-12-08T12:32:32Z
• Authors: Ahmet H. Elmaagacli; Michael Koldehoff; Johannes L. Zakrz...; Nina K. Steckel; Hellmut Ottinger; Dietrich W. Beelen
• Keywords: 0301 basic medicine; Leukemia; Reverse Transcriptase Polymerase Chain Reaction; Cell Cycle; Genes, myc; Anemia, Aplastic; Gene Expression; Antigens, CD34; Apoptosis; Immunophenotyping; 3. Good health; Gene Expression Regulation, Neoplastic; Repressor Proteins; 03 medical and health sciences; Leukemia, Megakaryoblastic, Acute; Case-Control Studies; Cell Line, Tumor; Proto-Oncogene Proteins; Humans; RNA Interference; Leukemia, Erythroblastic, Acute; RNA, Messenger; RNA, Small Interfering

Summary Growth factor‐independent 1B ( GFI1B ) is a transcription factor essential for the development and differentiation of erythroid megakaryocytic lineages. We evaluated expression in erythroleukaemia leukaemia, as well patients with other subtypes acute myeloid leukaemia (AML), lymphoblastic (ALL), chronic (CML), myelodysplastic syndrome (MDS), severe aplastic anaemia (SAA), myelofibrosis metaplasia (MMM) healthy volunteers. was increased at least threefold P < 0·01 compared controls) 0·05) their corresponding leukaemic cell lines HEL, K562, CMK M‐07e. Patients undifferentiated or monocytic AML, ALL, MMM, MDS CML had no significantly altered expression, whereas decreased 10‐fold SAA 0·0001 controls). Silencing by transfection small interfering RNA (siRNA) markedly reduced proliferation rate K562 NB4 0·01). Concomitantly, we observed two‐ to increase apoptosis these cells after siRNA towards . Our data indicate that plays major role AML‐M6 AML‐M7 qualifies target anti‐leukaemic strategies malignancies.