Mycobacterium boviswith different genotypes and from different hosts induce dissimilar immunopathological lesions in a mouse model of tuberculosis

• DOI: 10.1111/j.1365-2249.2009.03923.x
• Publication Date: 2009-02-25T10:55:07Z
• Authors: D Aguilar León; M J Zumárraga; R Jiménez Oropeza; A K Gioffré; A Bernardelli; H Orozco Estévez; A A Cataldi; R Hernández Pando
• Keywords: Swine Diseases; Mice, Inbred BALB C; 0303 health sciences; Granuloma; beta-Defensins; Virulence; Reverse Transcriptase Polymerase Chain Reaction; Tumor Necrosis Factor-alpha; Cattle Diseases; Genetic Variation; Nitric Oxide Synthase Type II; Mycobacterium bovis; 3. Good health; Colony-Forming Units Assay; Interferon-gamma; Mice; 03 medical and health sciences; Models, Animal; Disease Progression; Animals; Cattle; Lung; Tuberculosis, Pulmonary

Summary With the hypothesis that genetic variability of Mycobacterium bovis could influence virulence and immunopathology, five M. strains were selected from an epidemiological study in Argentina on basis their prevalence cattle occurrence other species. We then determined immunopathology evoked by these a well-characterized mouse model progressive pulmonary tuberculosis. The reference strain AN5 was used as control. BALB/c mice infected with this showed 50% survival after 4 months infection, moderate bacillary counts lung. Two weeks inoculation, it induced strong inflammatory response numerous granulomas pneumonia. In contrast, 04-303, isolated wild boar, most lethal its striking feature sudden pneumonia extensive necrosis. Strain 04-302, also boar but different spoligotype, similar pathology to lesser extent. 534, V2 (both cattle) 02-2B (from human) less virulent, permitting higher infection limited tissue damage. human isolates rapid, high stable expression interferon (IFN)-γ inducible nitric oxide synthase (iNOS). more virulent lower IFN-γ, tumour necrosis factor-α iNOS. Interestingly, very low murine beta defensin (mBD-4); whereas, control anti-microbial peptide, peaking at day 120. mBD-4 during early infection. Thus, reported clinical tuberculosis, variable virulence. This can be attributed induction pattern immune response.