In vitro water activity and pH dependence of mycelial growth and extracellular enzyme activities of Trichoderma strains with biocontrol potential*

• DOI: 10.1111/j.1365-2672.2004.02167.x
• Publication Date: 2004-12-27T02:59:28Z
• Authors: L. Kredics; L. Manczinger; Z. Antal; Z. Penzes; A. Szekeres; F. Kevei; E. Nagy
• Keywords: Trichoderma; Yeast extract

Aims: Water activity (aw) and pH are probably the most important environmental parameters affecting activities of mycoparasitic Trichoderma strains. Therefore it is to collect information on effects these factors mycelial growth in vitro extracellular enzymes involved nutrient competition (e.g. β‐glucosidase, cellobiohydrolase β‐xylosidase) mycoparasitism N‐acetyl‐β‐glucosaminidase, trypsin‐like protease chymotrypsin‐like protease) strains with biocontrol potential. Methods Results: dependence linear five examined belonging three different species groups was yeast extract soil media. Maximal rates were observed at aw 0·997 4·0 case all The values determined spectrophotometrically after incubation chromogenic p‐nitrophenyl p‐nitroaniline substrates. enzyme measured 0·950 for protease, 0·910 0·993 β‐xylosidase N‐acetyl‐β‐glucosaminidase enzymes. Optimal suggested be 5·0 3·0 β‐xylosidase, 6·0 between 7·0 activities, respectively. Conclusions: Extracellular able display under a wider range than those allowing growth. Significance Impact Study: Data about reveal useful applicability agricultural soils specific water relations.