Loss of tafazzin in yeast leads to increased oxidative stress during respiratory growth

• DOI: 10.1111/j.1365-2958.2008.06216.x
• Publication Date: 2008-04-10T10:39:43Z
• Authors: Shuliang Chen; Quan He; Miriam L. Greenberg
• Keywords: Paraquat; 0303 health sciences; Hot Temperature; Saccharomyces cerevisiae Proteins; Ethanol; Cardiolipins; Vitamin K 3; Hydrogen Peroxide; Saccharomyces cerevisiae; Protein Carbonylation; Oxidative Stress; 03 medical and health sciences; Reactive Oxygen Species; Acyltransferases; Oleic Acid

Summary The tafazzin ( TAZ ) gene is highly conserved from yeast to humans, and the taz1 null mutant shows alterations in cardiolipin (CL) metabolism, mitochondrial dysfunction stabilization of supercomplexes similar those found Barth syndrome, a human disorder resulting loss tafazzin. We have previously shown that Δ, which cannot remodel CL, ethanol‐sensitive at elevated temperature. In current report, we show response ethanol, CL mutants Δ as well crd1 synthesize exhibited increased protein carbonylation, an indicator reactive oxygen species (ROS). increase ROS most likely not due defective oxidant defence systems, do display sensitivity paraquat, menadione or hydrogen peroxide (H 2 O ). Ethanol carbonylation but can be rescued by supplementation with oleic acid, suggesting oleoyl‐CL and/or oleoyl‐monolyso‐CL enables growth ethanol decreasing oxidative stress. Our findings stress during respiratory may important implications for understanding pathogenesis syndrome.