Specific T cell unresponsiveness in human filariasis: diversity in underlying mechanisms

• DOI: 10.1111/j.1365-3024.1995.tb01002.x
• Publication Date: 2007-10-09T14:36:39Z
• Authors: ERLIYANI SARTONO; YVONNE C.M. KRUIZE; FELIX PARTONO; AGNES KURNIAWAN; RICK M. MAIZELS; MARIA YAZDANBAKHSH
• Keywords: Brugia malayi; Elephantiasis

In an attempt to overcome T cell unresponsiveness filarial antigens, 65 individuals belonging the three clinical groups of elephantiasis patients, microfilaraemics, and asymptomatic amicrofilaraemics who exhibited Brugia malayi adult worm antigen (BmA) were studied. Peripheral blood mononuclear cells cocultured with one following reagents that have been reported be effective in reconstituting proliferation: interleukin-2 (IL-2), interleukin-7 (IL-7), anti-interleukin-4, anti-interleukin-10, anti-CD2, anti-CD27, anti-CD28, indomethacin, phorbol myristate acetate (PMA), or calcium ionophore (A23187). We able antigen-specific only a minority Co-culture IL-2, IL-7, indomethacin PMA conditions which resulted enhanced proliferation BmA these individuals. general, patients was easier reverse than other groups: 50% 12.5% microfilaraemics 20% amicrofilaraemics. The results indicate more distinct immunological mechanism may account for exposed infected brugian filariasis.