Comparative microscopic and enzymatic characterization of the leaf necrosis induced in Arabidopsis thaliana by lead nitrate and by Xanthomonas campestris pv. campestris after foliar spray

• DOI: 10.1111/j.1365-3040.1995.tb00550.x
• Publication Date: 2006-04-30T14:11:24Z
• Authors: M. LUMMERZHEIM; M. SANDRONI; C. CASTRESANA; D. DE OLIVEIRA; M. VAN MONTAGU; D. ROBY; B. TIMMERMAN
• Keywords: Callose

ABSTRACT The heavy metal lead was administered to Arabidopsis thaliana plants by foliar spray. At a concentration of l4mol m −3 , the nitrate suspension induced densely distributed necrotic lesions on A. leaves. A number ecotypes were tested and differential response heavy‐metal toxicity noted. necrosis provoked as result phytotoxic effect had similar appearance observed in hypersensitive inoculation with Xanthomonas campestris pv. (Lummerzheim et al. 1993, Molecular Plant‐Microbe Interactions 6, 532–544). In addition this phenotypic resemblance, accumulation polyphenols callose depositions microscopic analysis, well increases activities stress‐related proteins β1,3‐glucanases, chitinases peroxidases, revealed significant similarities plant two treatments examined, bacterial infection. results allow establishment markers for both types stress.