Both Immunization with Protein and Recombinant Vaccinia Virus Can Stimulate CTL Specific for the E7 Protein of Human Papilloma Virus 16 in H‐2d Mice

• DOI: 10.1111/j.1365-3083.1995.tb03696.x
• Publication Date: 2006-07-01T04:13:35Z
• Authors: X. ZHU; M. TOMMASINO; K. VOUSDEN; E. SADOVNIKAVA; R. RAPPUOLI; L. CRAWFORD; M. KAST; C. J. M. MELIEF; P. C. L. BEVERLEY; H. J. STAUSS
• Keywords: CTL*; Recombinant virus; Poxviridae

The transforming protein E7 of human papilloma virus type 16 can stimulate cytotoxic T lymphocytes (CTL) which protect experimental animals against growth expressing tumour cells. In this study we compared CTL responses in mice immunized with either MF59 adjuvant or recombinant vaccinia (Vac‐E7). We have chosen H‐2 d because no E7‐specific been described MHC haplotype. Immunization these Vac‐E7 generated lysed target cells infected transfected the gene. from showed specificity for same Antibody blocking experiments revealed that both immunization and stimulated CD8 + effector CTL. find induced by two protocols was similar. A major epitope mapped to carboxyl terminal amino acids 48–98 protein. Peptide isolation followed HPLC separation indicated recognized purified peptide fractions. Together, suggests vaccines based on activate similar fine virus.