Advanced resources for plant genomics: a BAC library specific for the short arm of wheat chromosome 1B

• DOI: 10.1111/j.1365-313x.2006.02840.x
• Publication Date: 2006-08-15T12:00:37Z
• Authors: Jaroslav Janda; Jan Šafář; Marie Kubaláková; Jan Bartoš; Pavlína Kovářová; Pavla Suchánková; Stephanie Pateyron; Jarmila Číhalíková; Pierre Sourdille; Hana Šimková; Patricia Faivre‐R...; Eva Hřibová; Michel Bernard; Adam Lukaszewski; Jaroslav Doležel; Boulos Chalhoub
• Keywords: Bacterial artificial chromosome; genomic DNA

Summary Common wheat ( Triticum aestivum L., 2 n = 6 x 42) is a polyploid species possessing one of the largest genomes among cultivated crops (1C approximately 17 000 Mb). The presence three homoeologous (A, B and D), prevalence repetitive DNA make sequencing genome daunting task. We have developed novel ‘chromosome arm‐based’ strategy for to simplify this task; relies on sub‐genomic libraries large inserts. In paper, we used di‐telosomic line isolate six million copies short arm chromosome 1B (1BS) by flow sorting. Chromosomal was partially digested with Hin dIII construct an arm‐specific BAC library. library consists 65 280 clones average insert size 82 kb. Almost half (45%) has inserts larger than 100 kb, while 18% range in between 75 37% are shorter estimated coverage be 14.5‐fold, giving 99.9% probability identifying clone corresponding any sequence 1B. Each can sorted from appropriate cytogenetic stock, envisage that availability resources will greatly facilitate development ready‐to‐sequence physical maps map‐based gene cloning.