The immune response of oral epithelial cells induced by single‐species and complex naturally formed biofilms

• DOI: 10.1111/j.1399-302x.2009.00518.x
• Publication Date: 2009-06-15T16:42:41Z
• Authors: J. Eberhard; R. Pietschmann; W. Falk; S. Jepsen; H. Dommisch
• Keywords: Streptococcus mitis; Streptococcus oralis

In the oral cavity, surfaces are constantly exposed to a complex variety of microorganisms organized in biofilms. As part sophisticated local immune response, gingival epithelial cells (GECs) express antimicrobial peptides, such as human beta-defensin-2 (hBD-2), ribonuclease 7 (RNAase-7), and psoriasin (PSO), pro-inflammatory mediators, interleukin-8 (IL-8) 5-lipoxygenase (5-LO). The aim present study was test whether GECs show differential response single-species biofilms compared with multi-species biofilms.GECs were cultured from biopsies derived three different healthy donors (n = 3). To obtain naturally formed biofilm (NFB), polymer disks attached prostheses carried intraorally for 12, 24, 36, 48 h. addition, (SSB; Streptococcus mutans mitis) on vitro (12, h). messenger RNA (mRNA) expression hBD-2, RNAase-7, PSO, IL-8, 5-LO, glycerylaldehyde-3-phosphate dehydrogenase analysed using semi-quantitative reverse transcription-polymerase chain reaction.In GECs, hBD-2 mRNA significantly upregulated S. mitis-biofilm stimulation mutans-biofilm (P < 0.0001). contrast, RNAase-7 higher when responding both mitis 0.0001 P 0.001, respectively). IL-8 5-LO 0.0002, respectively).This found biofilm-dependent peptides inflammatory mediators GECs.