Heme oxygenase‐1 improves the survival of discordant cardiac xenograft through its anti‐inflammatory and anti‐apoptotic effects

• DOI: 10.1111/j.1399-3046.2007.00701.x
• Publication Date: 2007-05-10T19:57:07Z
• Authors: Xia Zhen‐Wei; Sun Jian‐Le; Qi Qi; Zhong Wen‐Wei; Zhang Xue‐Hong; Zhang Zi‐Li
• Keywords: Xenotransplantation; Hemin

Abstract: HO‐1 is a rate‐limiting enzyme in hemoglobin metabolism, and exerts anti‐inflammatory as well anti‐apoptotic effects. Previous studies have shown that expression of can prolong the survival concordant transplanted organs. However, little known about precise effect mechanism discordant xenotransplantation. In this study, we investigated role cardiac First, HUVECs were used to assess on TNF‐α‐induced apoptosis. Results showed TNF‐α induced apoptosis dose‐dependent manner. Moreover, induction by hemin suppressed action was reversed SnPP. The up‐regulation treatment significantly prolonged time xenograft, greatly reduced swelling myocardial cells, interstitial edema, lymphocyte infiltration, thrombus formation small vessels. Furthermore, overexpression attenuated serum level xenoantibody IgM, tissue deposition IgM complement 3 (C ) endangium. Finally, mitigated CD40L transcription xenograft recipient spleen. These results indicate improve inhibiting alleviating inflammation thrombosis.