Association of Piriformospora indica with Arabidopsis thaliana roots represents a novel system to study beneficial plant–microbe interactions and involves early plant protein modifications in the endoplasmic reticulum and at the plasma membrane

• DOI: 10.1111/j.1399-3054.2004.00424.x
• Publication Date: 2004-11-25T09:08:16Z
• Authors: Tatjana Peškan‐Be...; Bationa Shahollari; Pham Huong Giong; Solveig Hehl; Christine Markert; Verena Blanke; Gerhard Kost; Ajit Varma; Ralf Oelmüller
• Keywords: 0301 basic medicine; 03 medical and health sciences; 15. Life on land

Piriformospora indica , an endophytic fungus of the Sebacinaceae family, colonizes roots a wide variety plant species and promotes their growth, in manner similar to arbuscular mycorrhizal fungi. The results present study demonstrate that interacts also with non‐mycorrhizal host Arabidopsis thaliana its growth. interaction is detectable by appearance strong autofluorescence roots, followed colonization root cells fungal hyphae generation chlamydospores. Promotion growth was even before noticeable colonization. Membrane‐associated proteins from control after cultivation P. were separated two‐dimensional gel‐electrophoresis identified electrospray ionization mass spectrometry tandem spectrometry. Differences found expression glucosidase II, beta‐glucosidase PYK10, two glutathione‐ S ‐transferases several so‐far uncharacterized proteins. Based on conserved domains latter possible roles plant–microbe are predicted. Taken together, suggest powerful model system beneficial at molecular level. Furthermore, successful accommodation preceded protein modifications endoplasmatic reticulum as well plasma membrane host.