Cryopreservation Modifies Flow‐Cytometric Analysis of Hemopoietic Cells

• DOI: 10.1111/j.1423-0410.1995.tb02574.x
• Publication Date: 2009-03-06T04:09:45Z
• Authors: M. C. Rosillo; F. Ortuño; J. Rivera; J. M. Moraleda; V. Vicente
• Keywords: Buffy coat; Cryoprotectant

Abstract Although cryopreservation of human bone marrow has become very common in modern medicine, the knowledge on effects this procedure hemopoietic cells is still limited. We herein have investigated whether process concentrating to its buffy coat, exposure cryoprotectant dimethyl sulfoide (DMSO), and/or rate controlled freezing/thawing procedures modifies flow cytometric analysis cells. found that both DMSO freezing significantly relative proportions cell subsets and intensity expression certain surface antigens. Thus, percentages expressing CD7, CD13, CD33 CD34, were be lower cryopreserved coat merely exposed DMSO, comparison those untreated samples. Moreover, decreased further By contrast, CD19 antigen was higher last groups samples than or unfractionated marrow. Our present results suggest flow‐cytometric not fully equivalent corresponding fresh fractionated coat.