Blood group genotype analysis for the quality improvement of reagent test red blood cells

• DOI: 10.1111/j.1423-0410.2005.00623.x
• Publication Date: 2005-05-09T12:37:12Z
• Authors: A. Hult; Å. Hellberg; E. S. Wester; P. Olausson; J. R. Storry; M. L. Olsson
• Keywords: Zygosity

Background and Objectives Reagent red blood cells (RBCs) for antibody detection should express certain important antigens as a double dose, that is, the donors must be homozygous corresponding alleles. Traditionally, dose is determined by serological typing known allele frequencies. However, RHD zygosity cannot predicted serologically owing to absence of an antithetical antigen, FY confounded two variant haplotypes, * 0 X . Furthermore, lack reagents hampers our ability type some clinically antigen pairs such Do /Do b Materials Methods Genomic DNA was isolated from reagent RBC samples. Established, validated methods were used determine , DO genotypes. Results Three 52 D+ samples gave results differed genotype: presumed R 1 2 sample shown r′ r″ respectively. Five 59 homozygotes either A or B heterozygous, together with (three samples) (two samples). Seventy‐five tested A/A ( n = 14), A/B 39), B/B 22). Conclusions The show RhD Duffy phenotypes RBCs are unreliable we thought represented single dose. addition Dombrock genotyping provides information which useful in identification. We conclude selected genotype analyses valuable quality assurance measure ensure comply national international recommendations test sensitivity.