Anti‐T haemolysins: the effects of sialic acid removal and 2‐aminoethylisothiouronium bromide treatment of erythrocytes on immune lysis

• DOI: 10.1111/j.1423-0410.2010.01450.x
• Publication Date: 2010-12-01T00:49:22Z
• Authors: N. Burin des Roziers; G. Bodivit; P. Chadebech; R. Nzouakou; P. Bierling; F. Noizat‐Pirenne
• Keywords: Hemolysin

Background and Objectives Intravascular haemolytic reactions are reported in red‐cell T‐activated patients after blood transfusion. The relationship between T antigen antibodies present normal plasma these remains unclear. In this study, we assessed the activity of vitro comparison with anti‐A/B antibodies. Materials Methods We established a haemolysis assay based on treating target red‐blood‐cells (RBCs) 2‐aminoethylisothiouronium bromide (AET). Two hundred seven donor sera were analysed for anti‐T, haemolysins anti‐T agglutinins. Results Anti‐T found 4 (1·9%) using standard method 174 (84%) samples AET‐treated RBCs. Haemolysis correlated agglutination titres ( P < 10 −7 ). With both methods, much weaker than anti‐A anti‐B haemolysins. Gradual desialylation RBCs showed correlation sialic acid level as indicated by Sambucus nigra lectin mediated that was significantly increased (fold 2·4) independently expression. Conclusion These data indicate that, , anti‐T‐mediated depends primarily degree They suggest antibodies‐containing human is usually weak may only become significant very rare setting profound