The Amino Acid Sequence of the δ Subunit (Calmodulin) of Rabbit Skeletal Muscle Phosphorylase Kinase

• DOI: 10.1111/j.1432-1033.1981.tb05074.x
• Publication Date: 2005-03-03T20:57:41Z
• Authors: Roger J. A. GRAND; Shirish SHENOLIKAR; Philip COHEN
• Keywords: Cyanogen bromide; Phosphorylase kinase; Residue (chemistry)

The amino acid sequence of the phosphorylase kinase δ subunit has been determined using cyanogen bromide peptides. These peptides were ordered by isolation tryptic containing carboxy[ 14 C]methylmethionine. protein is identical to bovine uterus calmodulin and differs from brain only in amide assignments. contains 148 acids, including one residue trimethyllysine, a molecular weight 16680. N terminus blocked, probably with an acetyl group, net overall charge at pH 7 –24. asparagine residues 60 97 have shown be partially deamidated protein. N‐terminal tripeptide assignments on 58 not unequivocally.