Superoxide Release by Zymosan‐Stimulated Rat Kupffer Cells in vitro

• DOI: 10.1111/j.1432-1033.1981.tb05590.x
• Publication Date: 2005-03-03T21:19:32Z
• Authors: Rakesh BHATNAGAR; Rüdiger SCHIRMER; Martin ERNST; Karl DECKER
• Keywords: Zymosan

Kupffer cells were isolated from pronase‐perfused rat livers and maintained as a monolayer culture in state of high purity viability. Immediately after contact with zymosan particles, O 2 uptake the increased fivefold; about 50% net oxygen consumed was accounted for superoxide released into medium. Concomitantly, transient burst luminol‐dependent chemiluminescence, an activity NAD(P)H oxidase stimulation flow glucose through hexose monophophate shunt observed. Chemiluminescence production almost completely inhibited by dismutase iodoacetate. Zymosan‐induced chmiluminesceance not presence non‐penetrating thiol reagents, 5,5′‐dithio‐bis‐2‐nitrobenzoate iodoacetyl‐sepharose. Iodoacetate acted on cytosolic glucose‐6‐phosphate dehydrogenase rather than cell membrane.