Ascorbyl free radical as a reliable indicator of free‐radical‐mediated myocardial ischemic and post‐ischemic injury

• DOI: 10.1111/j.1432-1033.1990.tb19408.x
• Publication Date: 2005-03-04T04:41:15Z
• Authors: Sylvia PIETRI; Marcel CULCASI; Lucien STELLA; Patrick J. COZZONE
• Keywords: Ischemic injury

The real‐time kinetics of the release ascorbyl free radicals in coronary perfusate from isolated rat hearts submitted to an ischemia/reperfusion sequence has been achieved by continuous‐flow ESR using high‐speed acquisition techniques. Enhanced detection was obtained addition dimethyl sulfoxide (Me 2 SO), a strong cation chelator and oxidizing agent. A device allowed direct monitoring radical and/or ascorbate leakage observation doublet ( H = 0.188 mT g 2.0054). results showed that occurred mainly during sequences low‐flow ischemia (90 min) coupled or not with 30 min zero‐flow followed reperfusion (60 min). kinetic profiles ascorbyl‐free‐radical confirm quantitative terms expected correlation between duration ischemic insult magnitude extracellular upon reperfusion. There is indication depletion could be secondary oxygen‐derived‐free‐radical‐induced cellular damage. amount residual ascorbic acid quantitated on myocardial tissue at end Me SO as extracting solvent. Intense oxidation chemical stabilization resulting species provided measurement marked related ischemia. Perfusion superoxide dismutase first 10 greatly inhibited both endogenous depletion. These suggest redox system constitutes major protective mechanism against free‐radical‐induced injury. proposed perfusates extracts does require extensive preparation conditioning effluent samples. It provides interesting straightforward alternative evaluation detrimental processes affecting myocardium