The Tungsten Formylmethanofuran Dehydrogenase from Methanobacterium Thermoautotrophicum Contains Sequence Motifs Characteristic for Enzymes Containing Molybdopterin Dinucleotide

• DOI: 10.1111/j.1432-1033.1995.910_a.x
• Publication Date: 2004-12-22T21:22:24Z
• Authors: Andreas Hochheimer; Ruth A. Schmitz; Rudolf K. Thauer; Reiner Hedderich
• Keywords: Iron-Sulfur Proteins; Molybdenum; 0301 basic medicine; 0303 health sciences; Binding Sites; Base Sequence; Pteridines; Methanobacterium; Molecular Sequence Data; Coenzymes; Chromosome Mapping; Aldehyde Oxidoreductases; Molecular Weight; 03 medical and health sciences; Genes, Bacterial; Metalloproteins; Operon; Escherichia coli; Ferredoxins; Amino Acid Sequence; Cloning, Molecular; Molybdenum Cofactors; DNA Primers

Formylmethanofuran dehydrogenases are molybdenum or tungsten iron‐sulfur proteins containing a pterin dinucleotide cofactor. We report here on the primary structures of the four subunits FwdABCD of the tungsten enzyme from Methanobacterium thermoautotrophicum which were determined by cloning and sequencing the encoding genes fwdABCD. FwdB was found to contain sequence motifs characteristic for molybdopterin‐dinucleotide‐containing enzymes indicating that this subunit harbors the active site. FwdA, FwdC and FwdD showed no significant sequence similarity to proteins in the data bases. Northern blot analysis revealed that the four fwd genes form a transcription unit together with three additional genes designated fwdE, fwdF and fwdG. A 17.8‐kDa protein and an 8.6‐kDa protein, both containing two [4Fe‐4S] cluster binding motifs, were deduced from fwdE and fwdG. The open reading frame fwdF encodes a 38.6‐kDa protein containing eight binding motifs for [4Fe‐4S] clusters suggesting the gene product to be a novel polyferredoxin. All seven fwd genes were expressed in Escherichia coli yielding proteins of the expected size. The fwd operon was found to be located in a region of the M. thermoautotrophicum genome encoding molybdenum enzymes and proteins involved in molybdopterin biosynthesis.