Effects of Pancreatic Spasmolytic Polypeptide (PSP) on Epithelial Cell Function

• DOI: 10.1111/j.1432-1033.1996.00064.x
• Publication Date: 2004-08-31T15:30:15Z
• Authors: William R. Otto; Jagdish Rao; Helen M. Cox; Ewald Kotzian; Chung. Y. Lee; Robert A. Goodlad; Andrew Lane; Michael Gorman; Paul A. Freemont; Hans F. Hansen; Darryl Pappin; Nicholas A. Wright
• Keywords: Pancreatic function; Pancreatic polypeptide

Trefoil peptides are expressed near endodermal ulcerations and may modulate epithelial repair. The trefoil pancreatic spasmolytic polypeptide (PSP) was tested for growth activity in vitro on cells vivo following intragastric or intravenous infusion parenterally fed intact rats. Ion transport assessed as changes short‐circuit current rat intestine adenocarcinoma Ussing chambers. PSP stimulated of MCF‐7 Colo‐357 cells, but only the presence extracellular glutathione (GSH). effect attenuated by GSH depletion with buthionine sulphoximine, even GSH‐containing media. When GSH‐reduced carboxymethylated iodoacetic acid, it still depended its effect. Intestinal proliferation rats not affected either intraluminal infusion. had no basal ion flux jejunum monolayers. peptide did compete 125 I‐labeled epidermal factor receptor. [ 14 C]Iodoacetamide treatment PSP, followed prolonged tryptic digestion yielded predominantly a C‐labeled tetrapeptide fragment containing Cys104, lesser quantity 15‐amino‐acid Cys95 (molar ratio 15:1). reduce Cys6‐Cys104 terminal disulphide bond PSP. We conclude that some epithelia exhibit response to if is present. Reduction necessary this response, suggesting receptor signal transduction sensitive. could assist wound healing interactions exposed concurrently local high concentration.